THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Transfer the somatic cell nucleus into the enucleated egg cell.
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Isolate cells from the original (to be cloned) and an egg donor
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Remove and discard the nucleus from the egg cell.
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Implant the embryo into the surrogate mother.
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Detailed explanation-1: -Cloning using somatic cell nuclear transfer (SCNT) [ 1]. This procedure starts with the removal of the chromosomes from an egg to create an enucleated egg. The chromosomes are replaced with a nucleus taken from a somatic (body) cell of the individual or embryo to be cloned.
Detailed explanation-2: -Isolate the gene of interest-The first step is to isolate the gene of interest for cloning. This can be done by isolating the DNA from the cell that contains it and cutting it out with specific restriction enzymes. Restriction enzymes are bacterial enzymes that cut DNA at specific sequences.
Detailed explanation-3: -A virus or plasmid used in this way is known as a cloning vector, and the DNA propagated by insertion into it is said to have been cloned. To isolate a specific gene, one often begins by constructing a DNA library-a comprehensive collection of cloned DNA fragments from a cell, tissue, or organism.