THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Restriction Enzymes
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DNA Polymerase
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DNA Ligase
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RNA Polymerase
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Detailed explanation-1: -So the correct option is ‘Ligase’.
Detailed explanation-2: -Once they are joined by ligase, the fragments become a single piece of unbroken DNA. The target gene has now been inserted into the plasmid, making a recombinant plasmid. Once they are joined by ligase, the fragments become a single piece of unbroken DNA.
Detailed explanation-3: -DNA ligases are used to join the fragments of DNA generated by restriction enzymes. The availability of various types of restriction enzymes and ligases enables the transfer of specific DNA sequences from one molecule to another.
Detailed explanation-4: -Restriction Enzyme Digest Restriction enzymes are used to cut the DNA strands of two plasmids, producing DNA fragments with complementary sticky ends that can be reassembled to create a recombinant plasmid.
Detailed explanation-5: -Chapter Summary. Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence.