THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Mature mRNA does not contains introns.
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Mature mRNA directs the formation of the DNA.
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DNA taken from the nucleus is used to produced cDNA.
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Both A and C are true.
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Detailed explanation-1: -Hence, the correct answer is ‘By using mRNA, cDNA can be synthesized’
Detailed explanation-2: -No introns: Eukaryote genes commonly contain introns (non-coding sequences). These are removed after mRNA synthesis so cDNA contains no introns. This means that a cDNA copy of a gene can be isolated as a single, intron-free fragment.
Detailed explanation-3: -When scientists use viral enzymes to make cDNA from RNA isolated from the cells and tissues that they are studying, it does not contain introns due to being spliced out in mRNA. cDNA also does not contain any other gDNA that does not directly code for a protein (referred to as non coding DNA).
Detailed explanation-4: -The cDNA do not contain introns or the non-coding regions.-cDNA is produced by using an enzyme reverse transcriptase from RNA template by the process called reverse transcription.