AP BIOLOGY

Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.

Detailed explanation-2: -In prokaryotic cells, polymerase III is the major replicative polymerase, functioning in the synthesis both of the leading strand of DNA and of Okazaki fragments by the extension of RNA primers.

Detailed explanation-3: -One of the key molecules in DNA replication is the enzyme DNA polymerase. DNA polymerases are responsible for synthesizing DNA: they add nucleotides one by one to the growing DNA chain, incorporating only those that are complementary to the template.

Detailed explanation-4: -The isolated and purified DNA is treated with restriction endonucleases which cut the DNA into fragments. The restriction enzymes utilized in recombinant DNA technology are significant to detect the location at which the desired gene is introduced into the vector genome.