AP BIOLOGY

Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Detailed explanation-2: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

Detailed explanation-3: -Ligase is an enzyme which helps in joining the ends of DNA strands. Due to this reason, it is also called ‘genetic glue’.

Detailed explanation-4: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.

Detailed explanation-5: -Hint: DNA ligase is also called “molecular glue”. During DNA replication, It attaches the Okazaki fragments formed from the lagging strand during the replication of DNA. It is the enzymes that are commonly used in genetic engineering.