AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

DNA REPLICATION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
in gel electrophoresis, ____ charged DNA migrates toward the ____ end of the gel; ____ fragments travel further
A
positively, negative, smaller
B
positively, negative, larger
C
negatively, positive, smaller
D
negatively, positive, larger
Explanation: 

Detailed explanation-1: -The DNA molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole.

Detailed explanation-2: -Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode.

Detailed explanation-3: -The molecules travel through the pores in the gel at a speed that is inversely related to their lengths. This means that a small DNA molecule will travel a greater distance through the gel than will a larger DNA molecule.

Detailed explanation-4: -DNA (represented by circles) is loaded into the well of the gel at the end near the negative electrode. The DNA is pulled toward the positive electrode at the other end of the gel; how fast it moves depends on how big it is (bigger pieces of DNA make their way through the gel more slowly).

There is 1 question to complete.