AP BIOLOGY

Detailed explanation-1: -On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule.

Detailed explanation-2: -Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.

Detailed explanation-3: -The last deoxyribonucleotide is joined by a different enzyme, DNA ligase, which uses one ATP to join the Okazaki fragment into the growing lagging strand.

Detailed explanation-4: -The Okazaki fragments must be attached together into one continuous strand after replication. This is accomplished by the enzyme DNA ligase, which seals the sugar phosphate backbone of the Okazaki fragments. This allows for replication of two, continuous identical daughter strands of DNA.