AP BIOLOGY

Detailed explanation-1: -The ligase joins the two fragments of DNA to form a longer strand of DNA by “pasting” them together.

Detailed explanation-2: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair. Three human genes, LIG1, LIG3 and LIG4 encode ATP-dependent DNA ligases.

Detailed explanation-3: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Detailed explanation-4: -During DNA replication, It attaches the Okazaki fragments formed from the lagging strand during the replication of DNA. It is the enzymes that are commonly used in genetic engineering. Complete answer: DNA ligase is a biochemical catalyst which is used to join two strands of DNA mostly during the DNA synthesis.

Detailed explanation-5: -DNA ligases help bind Okazaki fragments that form on delayed strands as DNA replicates. Two DNA fragments can be bound by forming a phosphodiester bond between them with the help of energy molecules. The primary role of DNA ligase is to join two strands of DNA, which could be single or double strands.