AP BIOLOGY

Detailed explanation-1: -To unwind DNA, helicase first binds to DNA and then cleaves the hydrogen bonds connecting base pairs to separate the strands of the helix. Then single-stranded binding protein attaches to the single-stranded DNA. Helicase unwinds the DNA helix and SSB protein keeps the strands apart.

Detailed explanation-2: -Proteins called single-strand binding proteins coat the separated strands of DNA near the replication fork, keeping them from coming back together into a double helix.

Detailed explanation-3: -A protein called Helicase breaks the Hydrogen Bonds of the two strands of DNA forming a Replication Fork. Proteins called Binding Proteins keep the two DNA strands apart preserving the Replication Fork.

Detailed explanation-4: -As single-stranded DNA binding proteins are utilized in some of the most important aspects of DNA metabolism, they are used extensively in DNA replication, repair and recombination.

Detailed explanation-5: -Single-Stranded DNA Binding Protein (SSB) binds with high affinity in a cooperative manner to single-stranded DNA and does not bind well to double-stranded DNA. After binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily.