THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Detailed explanation-1: -The same restriction enzyme should be used when cutting out a gene of interest and cutting the plasmid vector where the DNA will be inserted so that the sticky ends of both will match up.
Detailed explanation-2: -The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.
Detailed explanation-3: -How do restriction enzymes work? Like all enzymes, a restriction enzyme works by shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.
Detailed explanation-4: -Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind.