AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
tools used in recombinant DNA technology
A
Target DNA, Taq polymerase, DNA cloning vector, host cell, modifying enzymes
B
Target DNA, restriction enzyme, DNA cloning vector, host cell, modifying enzyme
C
Target gene, restriction enzymes, DNA cloning vector, host cell, DNA polymerase
D
Target gene, restriction enzyme, DNA cloning vector, host cell, cDNA
Explanation: 

Detailed explanation-1: -The tools for plant rDNA technology include, vectors, restriction enzyme, ligation enzymes, bacterial hosts, methods to isolate and multiply nucleic acids, methods to quantify nucleic acids, Agrobacterium as a vector to insert foreign DNA into plants.

Detailed explanation-2: -Restriction enzymes can be isolated from bacterial cells and used in the laboratory to manipulate fragments of DNA, such as those that contain genes; for this reason they are indispensible tools of recombinant DNA technology (genetic engineering).

Detailed explanation-3: -Definition. A vector, as related to molecular biology, is a DNA molecule (often plasmid or virus) that is used as a vehicle to carry a particular DNA segment into a host cell as part of a cloning or recombinant DNA technique.

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