AP BIOLOGY

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THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Arrange the correct sequence 1-5 of the steps below.1-Adapter ligation by adding restriction sequence at both ends of cDNA2-mRNA isolated from human pancreatic cell3-Reverse transcriptase form first cDNA4-Ribonuclease degraded mRNA5-DNA polymerase form another strand of cDNA forming double stranded DNA
A
1, 2, 3, 4, 5
B
2, 3, 4, 5, 1
C
2, 4, 3, 1, 5
D
1, 3, 4, 5, 2
Explanation: 

Detailed explanation-1: -Reverse transcription reactions involve three main steps: primer annealing, DNA polymerization, and enzyme deactivation. The temperature and duration of these steps vary by primer choice, target RNA, and reverse transcriptase used.

Detailed explanation-2: -How many primers are used in the process of reverse transcriptase amplification? Explanation: The first primer of oligo – T is used during the reverse transcription of the mRNA to cDNA. The cDNA produced is a single stranded molecule thus synthesis of its complementary strand is necessary.

Detailed explanation-3: -Researchers present a novel approach to single-cell RNA sequencing (scRNA-seq) called “Live-seq”, which enables the extraction of mRNA from single cells without killing the cell. The method is published in Nature.

There is 1 question to complete.
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