THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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State the modifying enzyme that involved in gene cloning
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Restriction enzyme
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DNA Ligase
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RNA polymerase
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Peptidyl transferase
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Explanation:
Detailed explanation-1: -Different “enzymes are used in genetic engineering”. The most used enzymes are DNA polymerase, Reverse transcriptase, DNA ligase, restriction endonuclease, alkaline phosphatase. DNA polymerase functions in duplicating the content of DNA of a cell at the time of cell division.
Detailed explanation-2: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair.
Detailed explanation-3: -The enzyme used most often in experiments is T4 DNA ligase, which is purified from E. coli cells infected with bacteriophage T4.
There is 1 question to complete.