THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA cloning vector
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Host cell
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Modifying enzymes
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Restriction enzymes
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Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-2: -Both the plasmid and the target gene are (separately) digested with the restriction enzyme. The fragments are purified and combined. They have matching “sticky ends, ” or single-stranded DNA overhangs, so they can stick together.
Detailed explanation-3: -Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
Detailed explanation-4: -The gene and the plasmid are joined together using an enzyme called DNA ligase .