THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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They can be used to cleave mitochondrial DNA at specific restriction sites
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They can be used to cleave mitochondrial DNA at the random restriction sites
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They can be used to cleave nuclear DNA at specific restriction sites
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They can be used to cleave nuclear DNA at random restriction sites
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Detailed explanation-1: -Restriction enzymes cut DNA bonds between 3′ OH of one nucleotide and 5′ phosphate of the next one at the specific restriction site. Adding methyl groups to certain bases at the recognition sites on the bacterial DNA blocks the restriction enzyme to bind and protects the bacterial DNA from being cut by themselves.
Detailed explanation-2: -Restriction enzymes are used in biotechnology to cut DNA into smaller strands in order to study fragment length differences among individuals. This is referred to as restriction fragment length polymorphism (RFLP). They’re also used for gene cloning.
Detailed explanation-3: -Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
Detailed explanation-4: -Restriction enzyme cloning, or “restriction cloning, ” uses DNA restriction enzymes to cut a vector and an insert at specific locations so they can be easily joined together by the enzyme DNA ligase to create recombinant DNA.