AP BIOLOGY

Detailed explanation-1: -Phosphodiester bonds are formed by DNA ligase .

Detailed explanation-2: -DNA ligases catalyze the formation of phosphodiester bonds between adjacent 5′P and 3′OH ends in the backbone of double-stranded DNA and have a conserved core structure comprising the catalytic adenylation (AD) domain and an oligonucleotide-binding (OB) domain joined by a flexible linker region (1).

Detailed explanation-3: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

Detailed explanation-4: -A phosphodiester bond occurs when two of the hydroxyl groups in phosphoric acid react with hydroxyl groups on other molecules to form two ester bonds. Phosphodiester bonds are central to all life on Earth, [fn 1] as they make up the backbone of the strands of nucleic acid.

Detailed explanation-5: -During ligation both hydrogen ad covalent bonds form. Which bonds form first? Why do both types of bonds need to form? Hydrogen bonds form first.