THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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PovI
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SmaI
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BamHI
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EcoR2
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Detailed explanation-1: -BamHI (pronounced “Bam H one") (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site.
Detailed explanation-2: -EcoRI and BamHI cuts their respective restriction sites to form sticky ends while HaeIII and AluI cut DNA strands to form blunt ends. Q.
Detailed explanation-3: -BamHI binds at the recognition sequence 5’-GGATCC-3’, and cleaves these sequences just after the 5’-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long. In its unbound form, BamHI displays a central b sheet, which resides in between a helices .
Detailed explanation-4: -BamHI is a type II restriction enzyme derived from Bacillus amyloliquefaciens. Like all Type II restriction endonucleases, it is a dimer and the recognition site is palindromic and 6 bases in length. It recognizes the DNA sequence of G’GATCC and leaves an overhang of GATC which is compatible with many other enzymes.