THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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EcoRI restriction enzyme cuts plasmid at the following sites EXCEPT
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G and C bond
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Specific sequences
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Palindromic sequences
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Specific restriction sites
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Explanation:
Detailed explanation-1: -EcoRI recognizes the sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides.
Detailed explanation-2: -Restriction enzyme EcoRI cuts the DNA between bases G and A only when the sequence in DNA is.
Detailed explanation-3: -In the plasmid, the gene is now flanked by two EcoRI sites that were generated when the cut ends were ligated together.
Detailed explanation-4: -Note, after a reaction with the EcoRI enzyme, that the DNA of species A is cleaved into three fragments, corresponding to two EcoRI restriction sites, whereas that of species B is cleaved into four fragments, corresponding to three EcoRI restriction sites.
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