THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Examining the cells with an electron microscope
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Using radioactive tracers to locate plasmids
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Exposing the bacteria to an antibiotic that kills the cells lacking the plasmids
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Producing antibodies specific for each bacterium containing a recombinant plasmids
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Detailed explanation-1: -After a ligation, the next step is to transfer the DNA into bacteria in a process called transformation. Then, we can use antibiotic selection and DNA analysis methods to identify bacteria that contain the plasmid we’re looking for.
Detailed explanation-2: -Plasmids can be swapped between bacteria in a process called conjugation.
Detailed explanation-3: -What is Bacterial Transformation? Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneumoniae by Griffith in 1928.
Detailed explanation-4: -Transformation of environmental DNA can provide bacteria with a means to adapt quickly to a changing environment. While this can benefit microbes by facilitating the spread of antibiotic resistance, it can also be harmful if it causes the loss of beneficial alleles from a population.