AP BIOLOGY

Detailed explanation-1: -The ligase the most frequently used in molecular biology is the bacteriophage T4 DNA ligase. T4 DNA ligase is a 68 kDa monomer that requires Mg2+ and ATP as cofactors. T4 DNA ligase can connect blunt and cohesive ends, or repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Detailed explanation-2: -T4 DNA ligase requires ATP as a cofactor to catalyse phosphodiester bond formation during the polymerisation process. Herein, we describe the structure-activity relationship of ATP within the context of T4 DNA ligase-catalyzed oligonucleotide polymerisation.

Detailed explanation-3: -T4 DNA Ligase requires ATP as a cofactor.

Detailed explanation-4: -DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be discovered and is widely used in molecular biology, but its structure remained unknown.

Detailed explanation-5: -If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.