THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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T4 DNA polymerase
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T7 DNA polymerase
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Taq polymerase
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T7 RNA polymerase
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Detailed explanation-1: -Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. At its optimal temperature (72°C), nucleotides are incorporated at a rate of 2–4 kilobases per minute.
Detailed explanation-2: -Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol.
Detailed explanation-3: -DNA polymerase-a type of enzyme that synthesizes new strands of DNA complementary to the target sequence. The first and most commonly used of these enzymes isTaqDNA polymerase (fromThermis aquaticus), whereasPfuDNA polymerase (fromPyrococcus furiosus) is used widely because of its higher fidelity when copying DNA.
Detailed explanation-4: -Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus. It is used in PCR.