THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
Enzyme in plasmid
|
|
|
Enzyme that recognize and cut at specific base sequence
|
|
|
Enzyme that join okazaki fragment
|
|
|
Enzyme that join the plasmid and gene of interest
|
Detailed explanation-1: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.
Detailed explanation-2: -Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule-adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.
Detailed explanation-3: -Different restriction enzymes that recognize the same sequence are known as neoschizomers. These often cleave in different locales of the sequence. Different enzymes that recognize and cleave in the same location are known as isoschizomers.
Detailed explanation-4: -Restriction enzymes cut through both nucleotide strands, breaking the DNA into fragments, but they don’t always do this in the same way. SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end.