THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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i only
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i and ii only
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i, ii and iii only
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all of above
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Detailed explanation-1: -Plasmids contain three components: an origin of replication, a polylinker to clone the gene of interest (called multiple cloning site where the restriction enzymes cleave), and an antibiotic resistance gene (selectable marker).
Detailed explanation-2: -Ampicillin is commonly used as a selection marker since it binds to and inhibits the action of several enzymes that are involved in the synthesis of the cell wall. The ampicillin-resistant gene (ampR), on the other hand, catalyzes the hydrolysis of the B-lactam ring of ampicillin and naturally detoxifies the drug.
Detailed explanation-3: -Most plasmid vectors carry a short segment of lacZ gene that contains coding information for the first 146 amino acids of -galactosisdase. The host E. coli strains used are competent cells containing lacZ