THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To create the primers.
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To allow polymerase to create the complementary strands of DNA.
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To extend the time it takes to produce DNA.
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To allow substrates to copy DNA sequences.
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Detailed explanation-1: -The final stage is the extension step (20 sec to 1 min at 72 °C), which is performed so that the DNA polymerase extends the primer sequences from the 3’ of each primer to the end of the amplicon. A 1 min extension is typically sufficient to synthesize PCR fragments up to 2 kilobases (kb).
Detailed explanation-2: -What is the purpose of the Extension step of PCR? To allow polymerase to create the complementary strands of DNA.
Detailed explanation-3: -What is the purpose of the Extension step of PCR? To create the primers. To extend the time it takes to produce DNA. To allow polymerase to create the complementary strands of DNA.
Detailed explanation-4: -Taq polymerase Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus). T.
Detailed explanation-5: -Extension is achieved by using the loosened nucleotides of each base to grow the complementary DNA strand. The end result is two double-stranded products of DNA. The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.