AP BIOLOGY

Detailed explanation-1: -When a restriction endonuclease recognizes a sequence, it snips through the DNA molecule by catalyzing the hydrolysis (splitting of a chemical bond by addition of a water molecule) of the bond between adjacent nucleotides.

Detailed explanation-2: -One common method is based on restriction enzymes and DNA ligase. A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang.

Detailed explanation-3: -Recombinant DNA Technology If genomic DNA is isolated from one organism and cut with one particular restriction enzyme, a specific set of fragments can be separated and identified by electrophoresis. If DNA from a different organism is cut by the same restriction enzyme, a different set of fragments will be generated.

Detailed explanation-4: -Restriction enzyme, a type of endonuclease, functions by “inspecting” the length of a DNA sequence. Once it finds a recognition sequence, it binds and cut each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends.