THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
Plasmid
|
|
|
Cosmic
|
|
|
YAC
|
|
|
Bacteriophage
|
Detailed explanation-1: -Solution : Sall is a restriction enzyme isolated from Streptomyces albus.
Detailed explanation-2: -A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. They are often used as a cloning vector in genetic engineering. Cosmids can be used to build genomic libraries.
Detailed explanation-3: -The different types of vectors available for cloning are plasmids, bacteriophages, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs) and mammalian artificial chromosomes (MACs).
Detailed explanation-4: -So, the correct answer is ‘Single-stranded’
Detailed explanation-5: -pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4, 361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.