THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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2
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4
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8
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32
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Detailed explanation-1: -After two cycles, how many will we have? Well, a cycle of PcR doubles the DNA content because these two molecules are going to be split into four separate strands, and then each of the strands will be replicated. And so after a cycle you have four double stranded molecules And then after another cycle you have eight.
Detailed explanation-2: -Amplification of a DNA sequence typically involves 20-30 cycles of PCR and each cycle of PCR requires three temperature shifts.
Detailed explanation-3: -Complete answer: So, that means after the second cycle, it will produce 4 copies of DNA sample, then after the third cycle, 8 copies are produced, after the fourth cycle, 16 copies are produced, after the fifth cycle, 32 copies are produced, and lastly, after the sixth cycle, 64 copies of DNA samples are produced.
Detailed explanation-4: -The PCR process can amplify a single DNA to 2n times, where n is the number of cycles. Thus for 4 cycles of PCR, a given DNA template can be amplified to 16 duplicate strands.