AP BIOLOGY

Detailed explanation-1: -DNA extraction or Gene Isolation is the first step in the genetic engineering process. This is accomplished by taking a sample of bacteria containing the gene of interest and taking it through a series of steps that separate the DNA from the other parts of a cell.

Detailed explanation-2: -Genetic engineering is accomplished in three basic steps. These are (1) The isolation of DNA fragments from a donor organism; (2) The insertion of an isolated donor DNA fragment into a vector genome and (3) The growth of a recombinant vector in an appropriate host.

Detailed explanation-3: -To isolate a specific gene, one often begins by constructing a DNA library-a comprehensive collection of cloned DNA fragments from a cell, tissue, or organism. This library includes (one hopes) at least one fragment that contains the gene of interest.

Detailed explanation-4: -Gene of interest involves the structure needed for analysis that later recombined with the protein leading to the analysis, being held in the latter stages while translation occurs creating a genetically modified gene. Gene of interest is of significant importance while considering the gene cloning which is to occur.