THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The DNA must be cut only at one site near the gene of interest.
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The DNA must be cut at two sites to get a DNA fragment that contains gene of interest.
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The DNA must be cut at any two sites to get any DNA fragments from the organism.
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The DNA must not be cut at all so that the gene of interest is retained.
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Detailed explanation-1: -To isolate a specific gene, one often begins by constructing a DNA library-a comprehensive collection of cloned DNA fragments from a cell, tissue, or organism. This library includes (one hopes) at least one fragment that contains the gene of interest.
Detailed explanation-2: -The probes, which are mainly made up of DNA sequences are used to determine the gene of interest in a fragment of DNA. The DNA fragments containing the DNA probe are then amplified by the polymerase chain reaction (PCR), separated by gel electrophoresis, and transferred to nylon membranes through southern blotting.
Detailed explanation-3: -Genetic Engineering Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a region of DNA or adding a new segment of DNA.