AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
in producing insulin by genetic engineering, what is the first step to take?
A
isolate insulin from pancrease
B
isolate insulin mRNA from pancreas
C
isolate insulin DNA from pancreas
D
isolate insulin from animal tissue
Explanation: 

Detailed explanation-1: -The genetic engineering process The gene for human insulin is inserted into the gap in the plasmid. This plasmid is now genetically modified. The genetically modified plasmid is introduced into a new bacteria or yeast cell. This cell then divides rapidly and starts making insulin.

Detailed explanation-2: -Genetic engineering is accomplished in three basic steps. These are (1) The isolation of DNA fragments from a donor organism; (2) The insertion of an isolated donor DNA fragment into a vector genome and (3) The growth of a recombinant vector in an appropriate host.

Detailed explanation-3: -Insulin is prepared by recombinant DNA (rDNA) technology for medicinal purposes on a large scale.

Detailed explanation-4: -Scientists synthesized in the laboratory genes for the two insulin “A” and “B” chains. This is accomplished by chemically linking together small pieces of DNA sequence and then joining them in a specific manner to form complete genes.

There is 1 question to complete.