THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
What is enzyme X, process Y and process Z?i) Gene of interest and plasmid joined together by enzyme X.ii) Recombinant plasmid insert into host cell by process Y.iii) Identify bacteria with recombinant plasmid by process Z.
|
|
X:DNA Ligase, Y:Transformation, Z:Screening
|
|
|
X:Restriction endonuclease, Y:Transformation, Z:Amplification
|
|
|
X:DNA Ligase, Y:Insertion, Z:Screening
|
|
|
X:Restriction endonuclease, Y:Insertion, Z:Amplification
|
Explanation:
Detailed explanation-1: -Restriction enzymes and DNA ligase are often used to insert genes and other pieces of DNA into plasmids during DNA cloning.
Detailed explanation-2: -So, if the donor DNA and the vector DNA are both cut with the same enzyme, there is a strong possibility that the donor fragments and the cut vector will splice together because of the complementary overhangs. The resulting molecule is called recombinant DNA.
Detailed explanation-3: -The DNA is added to the vector by mixing the two together in the presence of the enzyme DNA ligase.
There is 1 question to complete.