THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Bacteriophage
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Vector
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Restriction enzyme
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Plasmid
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Detailed explanation-1: -Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species.
Detailed explanation-2: -A common method uses two types of enzymes: restriction enzymes and DNA ligase. A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site. Many restriction enzymes produce cut ends with short, single-stranded overhangs.
Detailed explanation-3: -In transformation, the recombinant DNA is inserted into a living cell, usually a bacterial cell. Changing an organism in this way is also called genetic engineering. Selection involves growing transformed bacteria to make sure they have the recombinant DNA.
Detailed explanation-4: -There are six steps involved in rDNA technology. These are – isolating genetic material, restriction enzyme digestion, using PCR for amplification, ligation of DNA molecules, Inserting the recombinant DNA into a host, and isolation of recombinant cells.