THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Can identify transformed bacteria that have recombinant plasmid
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Blue colonies shows bacteria consist of non-recombinant plasmid
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White colonies shows bacteria consist of recombinant plasmid
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Bacteria that have non-recombinant plasmid has disrupted lacZ gene
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Detailed explanation-1: -Final answer: Its DNA is as long as chromosomal DNA is not a correct statement about plasmids.
Detailed explanation-2: -The colonies formed by non-recombinant cells, therefore appear blue in color while the recombinant ones appear white.
Detailed explanation-3: -What is the purpose of the LacZ gene in a plasmid cloning vector? The LacZ gene is a selectable marker. Acts as a reporter gene which encodes beta-galctosidase. Expression of the lacz gene causes bacterial host cells carrying pUC18 to produce blue colonies when grown on medium containing a compound Xgal.
Detailed explanation-4: -Plasmids that are commonly used in gene cloning are about 3000 nucleotide pairs in length, have an amphicillin resistance gene and a gene that encodes the enzyme beta galactosidase. The gene encoding this enzyme is called the LacZ or blue color gene.