THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Restriction enzyme cleave DNA by cutting
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The covalent bond in the nitrogenous base
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The hydrogen bond between the nitrogenous base pair
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The glycosidic bond between the deoxyribose sugar and nitrogenous base
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The phosphodiester bond between the deoxyribose sugar and phosphate group
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Explanation:
Detailed explanation-1: -Restriction enzymes break phosphodiester bonds between the phosphate and the pentose sugar in sugar-phosphate backbone at the specific site.
Detailed explanation-2: -Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo, DNases or RNases, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes.
Detailed explanation-3: -Restriction enzymes cleave the phosphodiester bond between the phosphate and the pentose sugar at a particular location in the sugar-phosphate backbone.
Detailed explanation-4: -DNA ligases are enzymes that can form a phosphodiester bond at a single-strand break in DNA, a reaction between a 3′-OH group and a 5′-monophosphate.
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