THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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bacteria without plasmid will die
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Bacteria cells without any plasmid die because they are killed by x-gal
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Bacteria cells without any plasmid die because they are killed by the ampicilin
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that bacteria contain recombinant DNA
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Detailed explanation-1: -The bacterium cannot grow in the presence of the antibiotic ampicillin unless it contains the plasmid, and so there will be no growth on the LB/Amp plate of the bacteria without the plasmid.
Detailed explanation-2: -Bacteria without the plasmid are less likely to survive and reproduce. Some plasmids take extreme measures to ensure that they are retained within bacteria. For example, some carry a gene that makes a long-lived poison and a second gene that makes a short-lived antidote.
Detailed explanation-3: -Ampicillin is a penicillin antibiotic. It is used to treat many gram-positive and gram-negative bacteria.
Detailed explanation-4: -The answer is that bacterial cells can exist without plasmid DNA. The term “plasmid” refers to the extrachromosomal genetic component of the bacterial cell that is distinct from chromosomal DNA. These are little double-stranded, circular DNA molecules with the ability to replicate on their own.
Detailed explanation-5: -Ampicillin is an antibiotic used to selectively eliminate bacteria that have not been transformed with plasmids containing an ampicillin resistance gene.