AP BIOLOGY

Detailed explanation-1: -Sticky ends are helpful in cloning because they hold two pieces of DNA together so they can be linked by DNA ligase .

Detailed explanation-2: -What is the purpose of sticky ends? The purpose of sticky ends is to allow DNA to be cut and pasted back together. Sticky ends are single strands of DNA that overhang from the other side. They allow for DNA with a complementary sequence to easily be ligated into place.

Detailed explanation-3: -There is a higher chance of generating a recombinant DNA if you use a sticky-end cutter over a blunt-end cutter. Moreover, if you use a blunt-end cutter, you don’t have these single stranded overhangs to guide complementary regions of your insert and vector together in the orientation or configuration that you want.

Detailed explanation-4: -Sticky ends are mostly used for cloning technology where a DNA ligase is used to join two DNA fragments into one. Sticky ends facilitate ligation by DNA ligase by forming hydrogen bonds between complementary bases of the other strand. Thus, the sticky ends are better than the blunt ends.

Detailed explanation-5: -These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.