THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The DNA nucleotides are broken apart.
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The base-pairing rules for DNA are reversed.
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The double-stranded DNA is separated into two single strands of DNA.
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Detailed explanation-1: -During the first step in PCR, the starting solution is heated to the necessary temperature, usually between 90° and 100°C. As the heat builds, it breaks the bonds joining the two strands of the DNA double helix, thereby enabling the DNA to separate into two single strands.
Detailed explanation-2: -Denaturing stage The high temperature causes the hydrogen bonds? between the bases in two strands of template DNA to break and the two strands to separate. This results in two single strands of DNA, which will act as templates for the production of the new strands of DNA.
Detailed explanation-3: -The initial denaturation phase consists of a period at high temperature, during which the secondary structure of the complex double-stranded DNA (dsDNA) is melted to become single-stranded DNA (ssDNA).