THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The growth of the plasmid DNA
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DNA replication of plasmids outside a host cell
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The process of growth and division of the host cell
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Transformation of bacterial cells
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Detailed explanation-1: -The plasmid is introduced into bacteria via a process called transformation, and bacteria carrying the plasmid are selected using antibiotics. Bacteria with the correct plasmid are used to make more plasmid DNA or, in some cases, induced to express the gene and make protein.
Detailed explanation-2: -(1) Isolate the DNA sequence gene of interest. (2) Insert your DNA of interest into the vector plasmid: (a) cut both vector DNA and gene of interest with the same restriction enzymes, (b) mix the DNAs: they join by base pairing, (c) add DNA ligase to bond the pieces covalently. The result is a recombinant plasmid.
Detailed explanation-3: -…in recombinant DNA technology is amplification. This is carried out by inserting the recombinant DNA molecule into a bacterial cell, which replicates and produces many copies of the bacterial genome and the recombinant DNA molecule (constituting a DNA clone).
Detailed explanation-4: -Amplification is used for enhancing the copy number of a plasmid. Some plasmids have capability to replicate even in the absence of protein synthesis. This is incompatible with the primary bacterial chromosome, which are not able to replicate in the given environment.