AP BIOLOGY

Detailed explanation-1: -So the correct option is ‘Ligase’.

Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.

Detailed explanation-3: -Ligases join fragments of DNA together by catalysing the formation of bonds between neighbouring nucleotides.

Detailed explanation-4: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.

Detailed explanation-5: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair.