THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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recombinant DNA
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Target DNA
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plasmid
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ligase
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Detailed explanation-1: -Using recombinant DNA technology, scientists are able to create new DNA sequences that would not naturally exist under normal circumstances and environmental conditions. The resulting recombinant DNA is put into a host cell, where it is expressed into a new protein, which is the so-called recombinant protein.
Detailed explanation-2: -The added gene is called a transgene, which can be passed to progeny as a new component of the genome. The resulting organism carrying the transgene is called a transgenic organism or a genetically modified organism (GMO).
Detailed explanation-3: -A common method uses two types of enzymes: restriction enzymes and DNA ligase. A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site.
Detailed explanation-4: -When plasmids are used to produce a desired protein, the desired gene is inserted into the plasmid, and the plasmid is returned to the bacterium by transformation.