THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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is known as amplification.
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is heating a solution of DNA.
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uses restriction enzymes.
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isolate DNA molecules from the nucleus.
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Detailed explanation-1: -If genomic DNA is isolated from one organism and cut with one particular restriction enzyme, a specific set of fragments can be separated and identified by electrophoresis. If DNA from a different organism is cut by the same restriction enzyme, a different set of fragments will be generated.
Detailed explanation-2: -In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-3: -Restriction enzymes are a basic tool for biotechnology research. They are used for DNA cloning and DNA fingerprinting.
Detailed explanation-4: -Restriction Fragmentation DNA restriction enzymes break DNA strands at specific sites based on the nucleic acid sequence. Thus, digestion with a given restriction enzyme or combination of restriction enzymes will produce fragments of different lengths that are directly related to the DNA sequence.
Detailed explanation-5: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.