THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To screen for recombinant bacteria
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To join the recombinant DNA with the host cell
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To produce multiple copies of the desired gene
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To prevent the bacteria from reproducing
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Detailed explanation-1: -It is a technique used in PCR (polymerase chain reaction) to make multiple copies of the desired gene. It can precisely increase the copies of the target gene, without increasing the proportion of other genes. This amplification can either be an artificial or natural process.
Detailed explanation-2: -Cloning allows for the creation of multiple copies of genes, expression of genes, and study of specific genes.
Detailed explanation-3: -The efficacy of PCR is based on its ability to amplify a specific DNA segment through a pair of primers. The amplification capability of PCR allows the production of large amounts of specific DNA products, which can be detected by various methods (Erlich et al., 1991; Gibbs, 1991; Saiki et al., 1985).
Detailed explanation-4: -Gene amplification refers to an increase in the copy of a particular gene. Here, the number of copies of a gene increases without an increase in the number of copies of other genes. There may also be an increase in the gene products, i.e. RNA and protein.