THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Detailed explanation-1: -If EcoRI is used to digest both human chromosomal DNA and a plasmid, it will cut the chromosomal DNA over 700, 000 times (3 billion base pairs, cut every 4096 base pairs), but may only cut the plasmid once (5, 000 base pairs, cut every 4096 base pairs).
Detailed explanation-2: -To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
Detailed explanation-3: -When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes.
Detailed explanation-4: -Each restriction enzyme recognizes just one or a few restriction sites. When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.