AP BIOLOGY

Detailed explanation-1: –BamHI recognizes sequence 5’-G/GATCC-3’ and cleaves between the two G’s( shown by ‘ /’ in sequence) just after the 5’guanine on each strand.-This cleave will result in a 4 base pair with a long sticky end. So, the correct answer is, “5’-GGATCC-3’3’-CCTAGG-5’".

Detailed explanation-2: -GAATTC is recognition site of restriction endonuclease EcoR I. EcoR I is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoR I recognizes and cuts at the specific restriction site 5’-GAATTC-3’ and produces sticky ends.

Detailed explanation-3: -BamHI is a type II restriction enzyme derived from Bacillus amyloliquefaciens. Like all Type II restriction endonucleases, it is a dimer and the recognition site is palindromic and 6 bases in length. It recognizes the DNA sequence of G’GATCC and leaves an overhang of GATC which is compatible with many other enzymes.

Detailed explanation-4: -The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5’-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5’-GAATTC’-3 and will be cut in the same manner.