THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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involves joining together DNA molecules of 2 different sources to produce new genetic combinations
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usually involves plasmids (as vectors) and bacteria (as host cells)
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is a process to produce non-genetically identical copies of certain genes
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Detailed explanation-1: -In general, cloning vectors are plasmids that are used primarily to propagate DNA. They replicate in E. coli to high copy numbers and contain a multiple cloning site (also called a polylinker) with restriction sites used for inserting a DNA fragment.
Detailed explanation-2: -Plasmids are the most commonly used vectors for gene cloning. The type of plasmid used in the gene cloning has a single restriction site and, when cleaved by the same RE, gives rise to cohesive ends complementary to the DNA of interest. The enzyme ligase then forms the phosphodiester bond. 3.
Detailed explanation-3: -Bacterial cells are more commonly used as hosts for amplifying cloned DNA plasmids because they can produce a large quantity of DNA plasmids in a shorter amount of time. The plasmids can be easily isolated using commercially available plasmid DNA isolation kits.
Detailed explanation-4: -In bactofection-based gene therapy, the bacterium is considered the ‘vector’, which mediates carriage of the plasmid-based gene to the new host cell. Delivery of genetic material is achieved through entry of the entire bacterium into target cells.