THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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host cells
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cosmid
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restriction endonuclease
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DNA polymerase
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Detailed explanation-1: -Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.
Detailed explanation-2: -RT is used to synthesize complementary strand (cDNA) from mRNA template. It is also known as RNA dependent DNA polymerase.
Detailed explanation-3: -So, the correct option is ‘Introns’.
Detailed explanation-4: -The tools of recombinant DNA technology are the enzymes and other chemicals used to cut and join DNA molecules. These tools include restriction enzymes, DNA ligases, and polymerases.
Detailed explanation-5: -Recombinant DNA technology has also proven important to the production of vaccines and protein therapies such as human insulin, interferon and human growth hormone. It is also used to produce clotting factors for treating haemophilia and in the development of gene therapy.