THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Target DNA, Host Cell, Restriction Enzyme, Modifying Enzyme, ampicillin
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DNA Cloning Vector, Modifying Enzyme, cDNA, Host Cell, Restriction Enzyme
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Host Cell, X-gal, Target DNA, Restriction Enzyme, Modifying Enzyme
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Restriction Enzyme, Modifying Enzyme, Host Cell, DNA Cloning Vector, Gene of interest
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Detailed explanation-1: -They are two types, namely Endonucleases and Exonucleases. The Endonucleases cut within the DNA strand whereas the Exonucleases remove the nucleotides from the ends of the strands.
Detailed explanation-2: -Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.
Detailed explanation-3: -Restriction enzymes can be isolated from bacterial cells and used in the laboratory to manipulate fragments of DNA, such as those that contain genes; for this reason they are indispensible tools of recombinant DNA technology (genetic engineering).
Detailed explanation-4: -The type II restriction enzyme is commonly used in recombinant DNA technology.