AP BIOLOGY

Detailed explanation-1: -Restriction endonucleases are enzymes that cleave DNA molecules into smaller fragments.

Detailed explanation-2: -Many applications require conversion of genomic DNA into conveniently sized fragments by restriction endonuclease digestion. This yields DNA fragments of a convenient size for downstream manipulations. Restriction endonucleases are bacterial enzymes that bind and cleave DNA at specific target sequences.

Detailed explanation-3: -Restriction enzymes break phosphodiester bonds between the phosphate and the pentose sugar in sugar-phosphate backbone at the specific site.