THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Taq
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Vent
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Pfu
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Detailed explanation-1: -The error rate of Pfu DNA polymerase has been estimated at 1.3 x 10-6 mutations per bp per doubling using a PCR-based phenotypic assay9 (see lacI Phenotypic Mutation Assay below).
Detailed explanation-2: -The main difference between Pfu and alternative enzymes is that Pfu has superior thermostability and proof-reading properties. Unlike Taq DNA polymerase, Pfu DNA polymerase also possesses 3’->5’ exonuclease proofreading activity, resulting in PCR fragments with fewer errors than Taq-generated PCR inserts.
Detailed explanation-3: -With an error rate of 1 in 106–107 bases (Esteban et al. 1993), 29 DNA polymerase will be useful for amplification of genomic DNA or cDNA.
Detailed explanation-4: -The downside of Pfu is its speed which is slower than that of Taq.