AP BIOLOGY

Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Detailed explanation-2: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.

Detailed explanation-3: -A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using. No worries! We’ve got your back.

Detailed explanation-4: -DNA ligase works by catalyzing the formation of a phosphodiester bond between nucleotides on one strand of a double stranded DNA molecule. DNA ligase is able to create a covalent linkage between the 5’ phosphate group of one chain with the adjacent 3’-OH group of another.

Detailed explanation-5: -Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.